ONTOLOGY SOURCE REFERENCE
Term Source Name	"ncbitax"	"The MGED Ontology"	"ArrayExpress"	"NCI_thesaurus"	"mo"	"EFO"	"NCBITaxon"	"CCONT"	"BTO"	"XCO"	"CHEBI"	
Term Source File	"http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html"	"http://mged.sourceforge.net/ontologies/MGEDontology.php"	"http://www.ebi.ac.uk/arrayexpress"	"ncithesaurus.obo.alt"	"http://www.ebi.ac.uk/efo/"	""	"http://bioportal.bioontology.org/ontologies/47845"	"http://bioportal.bioontology.org/ontologies/47841"	"http://bioportal.bioontology.org/ontologies/50688"	"http://bioportal.bioontology.org/ontologies/50737"	"http://bioportal.bioontology.org/ontologies/50716"	
Term Source Version	""	""	""	""	""	""	"47845"	"47841"	"50688"	"50737"	"50716"	
Term Source Description	""	""	""	""	""	""	"National Center for Biotechnology Information (NCBI) Organismal Classification"	"Cell Culture Ontology"	"BRENDA Tissue and Enzyme Source Ontology"	"Experimental Conditions Ontology"	"Chemical Entities of Biological Interest Ontology"	
INVESTIGATION
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INVESTIGATION PUBLICATIONS
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STUDY
Study Identifier	"E-GEOD-424"
Study Title	"Transcription profiling time series of the response of Rattus norvegicus colon carcinoma cell line CC531 to treatment with butyrate or aspirin"
Study Description	"Temporal analysis of colon carcinoma cell line CC531 response to 4.5 mM butyrate or 3 mM aspirin. Samples taken at 2, 6, 12, 16 and 24 hours. The CC531 cell line has been widely used to study different aspects of tumor growth and metastasis and provides an excellent experimental platform to develop novel antitumor strategies. To characterize the CC531 model at the molecular level, we screened for mutations in genes covering important signal-transduction pathways that are known to play major roles during colon carcinogenesis, the wnt and the ki-ras signaling pathways. We found both a prototypic beta-catenin (Ctnnb1) mutation (Thr(41)Ile) and a ki-ras (G12D) mutation, providing unambiguous evidence for the constitutive activation of these pathways in CC531 cells. We further established comprehensive gene expression profiles of CC531 cells and investigated the molecular response to 2 antitumor drugs, butyrate and aspirin. Using oligonucleotide microarrays, we screened the expression levels of 7,700 genes and identified a total of 398 genes whose expression was significantly changed upon treatment with butyrate. When using aspirin, 121 genes were significantly altered. Interestingly, 36 genes were regulated by both butyrate and aspirin and 35 of them were regulated in the same direction. We found 7 differentially expressed genes, cyclin D1, cyclin E, c-myc, Fosl1, c-fos, Cd44 and follistatin, which are known targets of the beta-catenin and/or the ras pathway. In all cases, butyrate and aspirin reversed the changes in expression normally found in response to active signaling of these oncogenic pathways."
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Study Public Release Date	"2007-10-10"
Study File Name	"s_E-GEOD-424_study_samples.txt"
STUDY DESIGN DESCRIPTORS
Study Design Type	"transcription profiling by array"
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STUDY PUBLICATIONS
Study PubMed ID	"12800193"
Study Publication DOI	"10.1002/ijc.11215"
Study Publication Author List	"Anja Germann, Susanne Dihlmann, Manfred Hergenhahn, Magnus von Knebel Doeberitz, Robert Koesters"
Study Publication Title	"Expression profiling of CC531 colon carcinoma cells reveals similar regulation of beta-catenin target genes by both butyrate and aspirin."
Study Publication Status	"published"
Study Publication Status Term Accession Number	"EFO_0001796"
Study Publication Status Term Source REF	"EFO"
STUDY FACTORS
Study Factor Name	"dose"	"compound"	"time"
Study Factor Type	"dose"	"compound"	"time"
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STUDY ASSAYS
Study Assay File Name	"a_E-GEOD-424_GeneChip_assay.txt"
Study Assay Measurement Type	"transcription profiling"
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Study Assay Technology Type	"DNA microarray"
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STUDY PROTOCOLS
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STUDY CONTACTS
Study Person Last Name	"Germann"
Study Person First Name	"Anja"
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Study Person Email	"a.germann@dkfz-heidelberg.de"
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Study Person Address	"Division of Molecular Pathology, Department of Pathology, University Heidelberg, INF 220-221, Heidelberg, 69120, Germany"
Study Person Affiliation	"University Heidelberg"
Study Person Roles	"submitter"
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