ONTOLOGY SOURCE REFERENCE
Term Source Name	"ArrayExpress"	"EFO"	"XCO"	"CHEBI"	"NCBITaxon"	"OBI"	
Term Source File	"http://www.ebi.ac.uk/arrayexpress/"	"http://www.ebi.ac.uk/efo/efo.owl"	"http://bioportal.bioontology.org/ontologies/50737"	"http://bioportal.bioontology.org/ontologies/50716"	"http://bioportal.bioontology.org/ontologies/47845"	""	
Term Source Version	""	""	"50737"	"50716"	"47845"	""	
Term Source Description	""	""	"Experimental Conditions Ontology"	"Chemical Entities of Biological Interest Ontology"	"National Center for Biotechnology Information (NCBI) Organismal Classification"	""	
INVESTIGATION
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INVESTIGATION PUBLICATIONS
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STUDY
Study Identifier	"E-GEOD-29331"
Study Title	"Expression data from diclofenac-treated yeast cells"
Study Description	"Diclofenac is a widely used analgesic drug that can cause serious adverse drug reactions. We used Saccharomyces cerevisiae as model eukaryote to elucidate the molecular mechanisms of diclofenac toxicity and resistance. Although most yeast cells died during initial diclofenac treatment, some survived and started growing again. Microarray analysis of the adapted cells identified three major processes involved in diclofenac detoxification and tolerance. Especially pleiotropic drug resistance genes and genes under control of Rlm1p, a transcription factor in the protein kinase C (PKC) pathway, were upregulated in diclofenac-adapted cells. Genes involved in ribosome biogenesis and rRNA processing were downregulated, as well as zinc-responsive genes. Five independent yeast cultures were incubated with 100 µM diclofenac for 75 hours. Every 24h, cultures were diluted to OD600 ~0.1 in YNB containing 100 µM diclofenac. Five independent control cultures were grown in the absence of diclofenac."
Comment[Study Grant Number]	""
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Study Submission Date	""
Study Public Release Date	"2011-05-17"
Study File Name	"s_E-GEOD-29331_study_samples.txt"
STUDY DESIGN DESCRIPTORS
Study Design Type	"transcription profiling by array"
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STUDY PUBLICATIONS
Study PubMed ID	"21724882"
Study Publication DOI	"10.1128/AEM.00253-11"
Study Publication Author List	"van Leeuwen JS, Vermeulen NP, Vos JC"
Study Publication Title	"Involvement of the pleiotropic drug resistance response, protein kinase C signaling, and altered zinc homeostasis in resistance of Saccharomyces cerevisiae to diclofenac."
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STUDY FACTORS
Study Factor Name	"treatment"
Study Factor Type	"treatment"
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STUDY ASSAYS
Study Assay File Name	"a_E-GEOD-29331_GeneChip_assay.txt"
Study Assay Measurement Type	"transcription profiling"
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Study Assay Technology Type	"DNA microarray"
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STUDY PROTOCOLS
Study Protocol Name	"P-GSE29331-3"	"P-GSE29331-2"	"P-GSE29331-4"	"P-GSE29331-5"	"P-GSE29331-6"	"P-GSE29331-7"	"P-GSE29331-8"	"P-GSE29331-1"
Study Protocol Type	"grow"	"specified_biomaterial_action"	"nucleic_acid_extraction"	"labeling"	"hybridization"	"image_acquisition"	"feature_extraction"	"bioassay_data_transformation"
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Study Protocol Description	"An overnight culture of W303-1A was diluted to OD600=0.1 in YNB and divided over 10 tubes. Cultures were grown at 30°C and diluted every 24h to OD=0.1. 75 hours after first dilution, RNA was extracted."	"Cultures were grown in the presence of either 100 µM diclofenac or 0.1% DMSO (controls)."	"Qiagen RNeasy Mini Kit"	"Standard Affymetrix protocol, performed by microarray department (http://www.micro-array.nl/)."	"Standard Affymetrix protocol, performed by microarray department (http://www.micro-array.nl/)."	"Standard Affymetrix protocol, performed by microarray department (http://www.micro-array.nl/)."	"The data were analyzed with MAS 5.0 using default analysis settings."	"ID_REF =  VALUE = MAS5.0 signal intensity"
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STUDY CONTACTS
Study Person Last Name	"Vos"	"van Leeuwen"	"Vermeulen"	"Vos"
Study Person First Name	"J.C. "	"Jolanda"	"Nico"	"J"
Study Person Mid Initials	""	"S"	"P"	"C"
Study Person Email	"j.c.vos@vu.nl"	""	""	""
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Study Person Address	"Chemistry and Pharmaceutical Sciences, Vrije Universiteit, de Boelelaan 1083, Amsterdam, Netherlands"	""	""	""
Study Person Affiliation	"Vrije Universiteit"	""	""	""
Study Person Roles	"submitter"	""	""	""
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