ONTOLOGY SOURCE REFERENCE
Term Source Name	"CHEBI"	"BTO"	"EFO"	"NCBITAXON"	"NCIT"	"PR"	"XCO"	"UO"	
Term Source File	"http://data.bioontology.org/ontologies/CHEBI"	"http://data.bioontology.org/ontologies/BTO"	"http://data.bioontology.org/ontologies/EFO"	"http://data.bioontology.org/ontologies/NCBITAXON"	"http://data.bioontology.org/ontologies/NCIT"	"http://data.bioontology.org/ontologies/PR"	"http://data.bioontology.org/ontologies/XCO"	"http://data.bioontology.org/ontologies/UO"	
Term Source Version	"78"	"20"	"111"	"2"	"24"	"56"	"33"	"42"	
Term Source Description	"Chemical Entities of Biological Interest Ontology"	"BRENDA Tissue and Enzyme Source Ontology"	"Experimental Factor Ontology"	"National Center for Biotechnology Information (NCBI) Organismal Classification"	"National Cancer Institute Thesaurus"	"Protein Ontology"	"Experimental Conditions Ontology"	"Units of Measurement Ontology"	
INVESTIGATION
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INVESTIGATION PUBLICATIONS
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INVESTIGATION CONTACTS
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STUDY
Study Identifier	"E-GEOD-2676"
Study Title	"Transcriptional profiling of mouse synovial fibroblasts treated with indomethacin or iloprost [original title: Transcription profiling of synovial mouse fibroblast activated by IL-1beta effect of prostacyclin signaling]"
Study Description	"Synovial fibroblasts were extracted from knee joints of naíve mice (DBA/1J, male 8-12 week old), and passaged 3-4 times. Cells were plated at a concentration of 1 X 10^6 cells/dish in 60 mm dish in 5 ml of RPMI1640 medium with 1% heat-inactivated FCS at 37°C, 5% CO2. Cells were treated with vehicle or stimulated by IL-1beta. In IL-1beta (5ng/ml) stimulated groups, indomethacin (1 µM) and iloprost (1 µM) were further added either alone or in combination. After 6 hours of stimulation as described above, cells were isolated and frozen by liquid nitrogen, and stored at -80°C before use. Total RNA was extracted by RNAeasy Mini Kit. The RNA sample was labeled for hybridization onto the genechip array according to the standard affymetrix protocols."
Comment[Study Grant Number]	""
Comment[Study Funding Agency]	""
Study Submission Date	""
Study Public Release Date	"2008-06-12"
Study File Name	"s_E-GEOD-2676_study_samples.txt"
STUDY DESIGN DESCRIPTORS
Study Design Type	"compound_treatment_design"	"transcription profiling by array"
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STUDY PUBLICATIONS
Study PubMed ID	"16446378"
Study Publication DOI	"16446378"
Study Publication Author List	"Tetsuya Honda, Eri Segi-Nishida, Yoshiki Miyachi, Shuh Narumiya"
Study Publication Title	"Prostacyclin-IP signaling and prostaglandin E2-EP2/EP4 signaling both mediate joint inflammation in mouse collagen-induced arthritis."
Study Publication Status	"journal_article"
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STUDY FACTORS
Study Factor Name	"compound"
Study Factor Type	"compound"
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STUDY ASSAYS
Study Assay File Name	"a_E-GEOD-2676_GeneChip_assay.txt"
Study Assay Measurement Type	"transcription profiling"
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Study Assay Technology Type	"DNA microarray"
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STUDY PROTOCOLS
Study Protocol Name	"overall protocol"
Study Protocol Type	""
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Study Protocol Description	"DBA/1J_synovial fibroblast:  Synovial fibroblasts were extracted from knee joints of naíve mice (DBA/1J, male 8-12 week old), and passaged 3-4 times. Cells were plated at a concentration of 1 X 10^6 cells/dish in 60 mm dish in 5 ml of RPMI1640 medium with 1% heat-inactivated FCS at 37°C, 5% CO2.  Cells were treated vehicle or stimulated by IL-1beta. In IL-1beta (5ng/ml) stimulated groups, indomethacin (1 µM) and iloprost (1 µM) were further added either alone or in combination. After 6 hours of stimulation as described above, cells were isolated and frozen by liquid nitrogen, and stored at -80°C before use. Total RNA was extracted by RNAeasy Mini Kit. The  RNA sample was labeled for hybridization onto the genechip array according to the standard affymetrix protcols. Keywords = arthritis prostacyclin."
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STUDY CONTACTS
Study Person Last Name	"honda"
Study Person First Name	"tetsuya"
Study Person Mid Initials	""
Study Person Email	"t-honda@mfour.med.kyoto-u.ac.jp"
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Study Person Address	"kyoto university,  , kyoto, 606-8501, Japan"
Study Person Affiliation	"kyoto university"
Study Person Roles	"submitter"
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