ONTOLOGY SOURCE REFERENCE
Term Source Name	"The MGED Ontology"	"ArrayExpress"	"EFO"	"OBI"	"NCBITaxon"	"BTO"	"CHEBI"	
Term Source File	"http://mged.sourceforge.net/ontologies/MGEDontology.php"	"http://www.ebi.ac.uk/arrayexpress"	"http://www.ebi.ac.uk/efo/"	""	"http://bioportal.bioontology.org/ontologies/47845"	"http://bioportal.bioontology.org/ontologies/50231"	"http://bioportal.bioontology.org/ontologies/50447"	
Term Source Version	""	""	""	""	"47845"	"50231"	"50447"	
Term Source Description	""	""	""	""	"National Center for Biotechnology Information (NCBI) Organismal Classification"	"BRENDA Tissue and Enzyme Source Ontology"	"Chemical Entities of Biological Interest Ontology"	
INVESTIGATION
Investigation Identifier	""
Investigation Title	""
Investigation Description	""
Investigation Submission Date	""
Investigation Public Release Date	""
Comment [Created with configuration]	""
Comment [Last Opened With Configuration]	""
INVESTIGATION PUBLICATIONS
Investigation PubMed ID	""
Investigation Publication DOI	""
Investigation Publication Author List	""
Investigation Publication Title	""
Investigation Publication Status	""
Investigation Publication Status Term Accession Number	""
Investigation Publication Status Term Source REF	""
INVESTIGATION CONTACTS
Investigation Person Last Name	""
Investigation Person First Name	""
Investigation Person Mid Initials	""
Investigation Person Email	""
Investigation Person Phone	""
Investigation Person Fax	""
Investigation Person Address	""
Investigation Person Affiliation	""
Investigation Person Roles	""
Investigation Person Roles Term Accession Number	""
Investigation Person Roles Term Source REF	""
STUDY
Study Identifier	"E-GEOD-15799"
Study Title	"Adenocarcinoma cells treated with the NSAID NS-398 [original title: Expression data from NS398-treated and control HT29 colon adenocarcinoma cell line samples]"
Study Description	"The whole-genome oligonucleotide microarray analysis of NS398-treated HT29 colon adenocarcinoma cells samples can give an insight into global molecular background of selective COX2 inhibitor administration in order to find other target molecules and pathways influenced by NS398 selective COX2 inhibitor treatment in the epithelial cells. Total RNA was extracted from NS398-treated and control HT29 colon adenocarcinoma cells and hybridized on Affymetrix HGU133 Plus 2.0 microarrays"
Comment[Study Grant Number]	""
Comment[Study Funding Agency]	""
Study Submission Date	""
Study Public Release Date	"2010-03-16"
Study File Name	"s_E-GEOD-15799_study_samples.txt"
STUDY DESIGN DESCRIPTORS
Study Design Type	"transcription profiling by array"
Study Design Type Term Accession Number	""
Study Design Type Term Source REF	""
STUDY PUBLICATIONS
Study PubMed ID	"20087348"
Study Publication DOI	"20087348"
Study Publication Author List	"Galamb O, Spisák S, Sipos F, Tóth K, Solymosi N, Wichmann B, Krenács T, Valcz G, Tulassay Z, Molnár B"
Study Publication Title	"Reversal of gene expression changes in the colorectal normal-adenoma pathway by NS398 selective COX2 inhibitor."
Study Publication Status	"journal_article"
Study Publication Status Term Accession Number	""
Study Publication Status Term Source REF	"The MGED Ontology"
STUDY FACTORS
Study Factor Name	"treatment"
Study Factor Type	"treatment"
Study Factor Type Term Accession Number	""
Study Factor Type Term Source REF	""
STUDY ASSAYS
Study Assay File Name	"a_E-GEOD-15799_GeneChip_assay.txt"
Study Assay Measurement Type	"transcription profiling"
Study Assay Measurement Type Term Accession Number	""
Study Assay Measurement Type Term Source REF	""
Study Assay Technology Type	"DNA microarray"
Study Assay Technology Type Term Accession Number	""
Study Assay Technology Type Term Source REF	""
Study Assay Technology Platform	""
STUDY PROTOCOLS
Study Protocol Name	"P-GSE15799-2"	"P-GSE15799-3"	"P-GSE15799-4"	"P-GSE15799-5"	"P-GSE15799-6"	"P-GSE15799-1"
Study Protocol Type	"nucleic_acid_extraction"	"labeling"	"hybridization"	"image_aquisition"	"feature_extraction"	"bioassay_data_transformation"
Study Protocol Type Term Accession Number	""	""	""	""	""	""
Study Protocol Type Term Source REF	""	""	""	""	"The MGED Ontology"	"The MGED Ontology"
Study Protocol Description	"RNeasy Mini extraction of total RNA was performed according to the manufacturer instructions"	"Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 micrograms of total RNA"	"Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45C on Affymetrix HGU133 Plus 2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450"	"GeneChips were scanned using the GeneChip 3000 Scanner"	"The data were preprocessed by the function mas5 from the Bioconductor affy package"	"ID_REF = <br>VALUE = MAS5-calculated Signal intensity<br>ABS_CALL = the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)<br>DETECTION P-VALUE = p-value that indicates the significance level of the detection call"
Study Protocol URI	""	""	""	""	""	""
Study Protocol Version	""	""	""	""	""	""
Study Protocol Parameters Name	""	""	""	""	""	""
Study Protocol Parameters Name Term Accession Number	""	""	""	""	""	""
Study Protocol Parameters Name Term Source REF	""	""	""	""	""	""
Study Protocol Components Name	""	""	""	""	""	""
Study Protocol Components Type	""	""	""	""	""	""
Study Protocol Components Type Term Accession Number	""	""	""	""	""	""
Study Protocol Components Type Term Source REF	""	""	""	""	""	""
STUDY CONTACTS
Study Person Last Name	"Sipos"	"Galamb"	"Barna"	"Spisak"	"Molnar"
Study Person First Name	"Ferenc"	"Orsolya"	"Wichmann"	"Sandor"	"Bela"
Study Person Mid Initials	""	""	""	""	""
Study Person Email	""	""	"wwbarna@yahoo.com"	""	""
Study Person Phone	""	""	""	""	""
Study Person Fax	""	""	""	""	""
Study Person Address	""	""	"SOTE, Szentkirály, Budapest, Hungary"	""	""
Study Person Affiliation	""	""	"SOTE"	""	""
Study Person Roles	""	""	"submitter"	""	""
Study Person Roles Term Accession Number	""	""	""	""	""
Study Person Roles Term Source REF	""	""	""	""	""
Comment[Study Person REF]	""	""	""	""	""